Naked-eye LAMP protocol enables rapid Chikungunya virus detection
The study reports a laboratory protocol for naked-eye visual detection of Chikungunya virus using capillary-modified loop-mediated isothermal amplification (LAMP). Researchers amplified viral RNA extracted from cultured virus at 63°C for 25 minutes, with a distinct yellowish-green color change confirming amplification. The method demonstrates how isothermal amplification can be adapted for simple, instrument-free readouts. This protocol offers a potential framework for decentralized testing in resource-limited settings where Chikungunya outbreaks occur, though clinical validation against patient samples is required before diagnostic deployment.
The original study
Naked-Eye Visual Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Chikungunya Virus.
- Authors
- Wang J, Zhao J, Zhang L
- Journal
- Methods in molecular biology (Clifton, N.J.)
- Type
- Journal Article
- PMID
- 42734785
Original abstract
Chikungunya virus (CHIKV) represents a significant global health threat due to its recent resurgence. This protocol highlights the use of capillary-modified visual loop-mediated isothermal amplification (LAMP) technology for the rapid detection of CHIKV. We employed Vero cells for virus propagation and utilized the MolPure Viral DNA/RNA Kit for nucleic acid extraction, successfully amplifying extracted viral RNA at 63°C for 25 min. The presence of CHIKV was indicated by a distinct yellowish-green color change, demonstrating the effectiveness and simplicity of this protocol. Our protocol underscores the potential of LAMP technology as a rapid diagnostic tool for CHIKV, enabling timely public health interventions.