Selux Next-Generation Phenotyping System Delivers Rapid AST Results in Under 6 Hours
A multicenter evaluation of the Selux NGP antimicrobial susceptibility testing system demonstrated excellent performance against reference broth microdilution, with very major and major error rates below 1% and essential and categorical agreement above 95%. The system produced results in an average of 5.65 hours from assay start, offering a significant speed advantage over conventional phenotypic methods.
The original study
Multicenter evaluation of the Selux Next-Generation Phenotyping antimicrobial susceptibility testing system.
- Authors
- Baker KR, Flentie K, Spears BR, Mozharov S, Roberts K, El Ganbour A, et al.
- Journal
- Journal of clinical microbiology
- Type
- Multicenter Study, Journal Article, Research Support, Non-U.S. Gov't
- PMID
- 38051069
Original abstract
The Selux Next-Generation Phenotyping (NGP) system (Charlestown, MA) is a new antimicrobial susceptibility testing system that utilizes two sequential assays performed on all wells of doubling dilution series to determine MICs. A multicenter evaluation of the performance of the Selux NGP system compared with reference broth microdilution was conducted following FDA recommendations and using FDA-defined breakpoints. A total of 2,488 clinical and challenge isolates were included; gram-negative isolates were tested against 24 antimicrobials, and gram-positive isolates were tested against 15 antimicrobials. Data is provided for all organism-antimicrobial combinations evaluated, including those that did and did not meet FDA performance requirements. Overall very major error and major error rates were less than 1% (31/3,805 and 107/15,606, respectively), essential agreement and categorical agreement were >95%, reproducibility was ≥95%, and the average time-to-result (from time of assay start to time of MIC result) was 5.65 hours.