BD Max Open System Validated for Multiplex Mycobacterial Detection Directly from Clinical Specimens
A multiplex real-time PCR assay validated on the BD Max open-access platform enables simultaneous detection and differentiation of M. tuberculosis complex, M. avium complex, and Mycobacterium spp. directly from clinical samples. The automated extraction-to-result workflow showed high sensitivity and specificity compared to conventional culture methods. This approach allows laboratories already using the BD Max platform to add mycobacterial testing with minimal additional investment in equipment or training.
The original study
Validation of a Multiplex Real-Time PCR Assay for Detection of Mycobacterium spp., Mycobacterium tuberculosis Complex, and Mycobacterium avium Complex Directly from Clinical Samples by Use of the BD Max Open System.
- Authors
- Rocchetti TT, Silbert S, Gostnell A, Kubasek C, Widen R
- Journal
- Journal of clinical microbiology
- Type
- Journal Article, Validation Study
- PMID
- 27008873
Original abstract
A multiplex real-time PCR was validated on the BD Max open system to detect different Mycobacterium tuberculosis complex, Mycobacterium avium complex, and Mycobacterium spp. directly from clinical samples. The PCR results were compared to those with traditional cultures. The multiplex PCR assay was found to be a specific and sensitive method for the rapid detection of mycobacteria directly from clinical specimens.