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DYNAMIC-III: ctDNA-Guided Therapy De-Escalation Reduces Oxaliplatin Use in Stage III Colon Cancer

This landmark phase 2/3 randomized trial in 968 stage III colon cancer patients validated ctDNA as a strong prognostic classifier, with ctDNA-negative patients having significantly better three-year recurrence-free survival (87% vs 49%). ctDNA-guided de-escalation reduced oxaliplatin use from 89% to 35% and lowered hospitalization rates, though recurrence-free survival narrowly missed non-inferiority. For ctDNA-positive patients, chemotherapy escalation did not improve outcomes, and persistent ctDNA after treatment predicted dismal prognosis (three-year RFS 14%), underscoring the need for novel strategies beyond conventional chemotherapy intensification.

The original study

Circulating tumor DNA-guided adjuvant therapy in locally advanced colon cancer: the randomized phase 2/3 DYNAMIC-III trial.

Authors
Tie J, Wang Y, Loree JM, Cohen JD, Wong R, Price T, et al.
Journal
Nature medicine
Type
Journal Article, Randomized Controlled Trial, Clinical Trial, Phase III, Clinical Trial, Phase II, Multicenter Study
PMID
41115959
Read the original study →

Original abstract

Adjuvant chemotherapy in stage III colon cancer provides uncertain benefit at the individual level. Circulating tumor DNA (ctDNA) may help refine risk-adjusted treatment selection. In this multicenter, randomized, phase 2/3 trial, patients with stage III colon cancer underwent ctDNA testing 5-6 weeks after surgery and were assigned (1:1) to ctDNA-guided or standard management. In the ctDNA-guided arm, patients negative for ctDNA received de-escalated therapy, whereas ctDNA-positive patients received escalated therapy. Clinicians prespecified the standard regimen. Primary endpoints were 3-year recurrence-free survival (RFS) for ctDNA-negative patients and 2-year RFS for ctDNA-positive patients. Secondary endpoints included treatment-related hospitalization and ctDNA clearance. Among 968 evaluable patients, 702 (72.5%) were ctDNA negative. With a median follow-up of 47 months, ctDNA-negative patients experienced significantly fewer recurrences than ctDNA-positive patients (3-year RFS 87% versus 49%; P < 0.001). In ctDNA-negative patients, de-escalation reduced oxaliplatin use (34.8% versus 88.6%) and hospitalizations (8.5% versus 13.2%) but yielded slightly lower RFS than standard management (85.3% versus 88.1%), not meeting the non-inferiority margin. In ctDNA-positive patients, higher ctDNA burden correlated with recurrence risk (3-year RFS 77% to 23% across quartiles; P < 0.001). Escalated therapy did not improve outcomes over standard management (2-year RFS 51% versus 61%). There was no unexpected toxicity. Persistent ctDNA after treatment predicted markedly worse prognosis (3-year RFS 14% versus 79%). ctDNA is validated as a strong prognostic classifier. ctDNA-guided de-escalation reduced oxaliplatin exposure and adverse events with outcomes approaching standard of care, whereas exploratory chemotherapy intensification conferred no RFS benefit, suggesting a need for novel strategies in ctDNA-positive disease.Australian New Zealand Clinical Trials Registry Identifier: ACTRN12617001566325 .