Liquid Biopsy Significance 7/10

Zanubrutinib-Venetoclax-Obinutuzumab Achieves 85% Undetectable MRD in Relapsed CLL

The CLL2-BZAG phase 2 trial demonstrated that MRD-guided zanubrutinib, venetoclax, and obinutuzumab induced deep remissions in 40 relapsed/refractory CLL patients, including 45% previously exposed to BTK inhibitors. Best undetectable MRD rate reached 85%, with estimated 18-month progression-free and overall survival both at 96%. This trial extends the evidence for MRD-guided fixed-duration triple combinations in CLL and is notable for including patients previously treated with both BTK inhibitors and venetoclax.

The original study

MRD-guided zanubrutinib, venetoclax, and obinutuzumab in relapsed CLL: primary end point analysis from the CLL2-BZAG trial.

Authors
Fürstenau M, Robrecht S, Schneider C, Tausch E, Giza A, Ritgen M, et al.
Journal
Blood
Type
Journal Article, Clinical Trial, Phase II, Multicenter Study
PMID
39883943
Read the original study →

Original abstract

The phase 2 CLL2-BZAG trial tested a measurable residual disease (MRD)-guided combination treatment of zanubrutinib, venetoclax, and obinutuzumab after an optional bendamustine debulking in patients with relapsed/refractory chronic lymphocytic leukemia (CLL). In total, 42 patients were enrolled and 2 patients with ≤2 induction cycles were excluded from the analysis population per protocol. Patients had a median of 1 prior therapy (range, 1-5); 18 patients (45%) had already received a Bruton tyrosine kinase (BTK) inhibitor (BTKi); 7 patients (17.5%) venetoclax; and, of these, 5 (12.5%) had received both. Fifteen patients (37.5%) had a TP53 mutation/deletion, and 31 (77.5%) had unmutated immunoglobulin heavy chain variable region gene. With a median observation time of 21.5 months (range, 8.0-35.3) the most common adverse events were COVID-19 (n = 26 patients), diarrhea (n = 15), infusion-related reactions (n = 15), thrombocytopenia (n = 14), nausea (n = 12), fatigue (n = 12), and neutropenia (n = 12). Two patients had fatal adverse events (COVID-19, and fungal pneumonia secondary to COVID-19). After 6 months of the triple combination, all patients responded, and 21 (52.5%; 95% confidence interval, 36.1-68.5) showed undetectable MRD (uMRD) in the peripheral blood. In many patients, remissions deepened over time, with a best uMRD rate of 85%. The estimated progression-free and overall survival rates at 18 months were 96% and 96.8%, respectively. No patient has yet required a subsequent treatment. In summary, the MRD-guided triple combination of zanubrutinib, venetoclax, and obinutuzumab induced deep remissions in a relapsed CLL population enriched for patients previously treated with a BTKi/venetoclax. This trial was registered at www.clinicaltrials.gov as #NCT04515238.