CRISPR in Cancer: From Functional Genomics to Diagnostics and Therapeutics
This comprehensive review in Nature Reviews Cancer surveys the transformative impact of CRISPR technology across cancer research, from dissecting cancer genetics and the noncoding genome to uncovering therapeutic vulnerabilities through high-throughput screens. The authors discuss how CRISPR tools have enabled new approaches to understanding tumour heterogeneity and are being adapted for improved cancer diagnosis and treatment. The review highlights the transition from research tool to clinical platform and outlines future directions for CRISPR-based cancer applications.
The original study
CRISPR in cancer biology and therapy.
- Authors
- Katti A, Diaz BJ, Caragine CM, Sanjana NE, Dow LE
- Journal
- Nature reviews. Cancer
- Type
- Journal Article, Review, Research Support, N.I.H., Extramural
- PMID
- 35194172
Original abstract
Over the past decade, CRISPR has become as much a verb as it is an acronym, transforming biomedical research and providing entirely new approaches for dissecting all facets of cell biology. In cancer research, CRISPR and related tools have offered a window into previously intractable problems in our understanding of cancer genetics, the noncoding genome and tumour heterogeneity, and provided new insights into therapeutic vulnerabilities. Here, we review the progress made in the development of CRISPR systems as a tool to study cancer, and the emerging adaptation of these technologies to improve diagnosis and treatment.